11:47 Lab 6-Sugars and Enzyme Specif 1) What common monosaccharide will be produ
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11:47 Lab 6-Sugars and Enzyme Specif 1) What common monosaccharide will be produced by the hydrolysis of both sucrose and lactose? Explain your answer. (1 pt 2) Based on the results of the glucose test strips, under what reaction conditions dd hydrolysis of sucrose occur? How do you know? (1 pr) 3) Based on the results of the glucose test strips, under what reaction conditions did hydrolysis of lactose occur? How do you know? (1 pt Last modified 2/17/2018 What do the results of the glucose test strips suggest about the enzyme specificity of invertase and lactase? (1 pt 5) Discuss the degree of agreement between the glucose test strip results and the Benedict's test results. If there were any disagreements, please explain what may have caused them. (1 pt) 6) Why did one of the disaccharides react with the Benedict's test, even without being hydrolyzed? Explain using chemical terms (1 2 of 2Explanation / Answer
1) glucose since both contain glucose as one of the monosaccharide
2) acidic conditions using a mineral acid and enzyme invertase
3) mostly neutral conditions using an enzyme lactase
4) Both the enzymes are very specific. Invertase catalyzes hydrolysis of sucrose whereas lactase that of lactose
6) Disaccarides such as lactose and maltose are reducing sugars due to an OH group attached to the anomeric carbon which can tautomerize, but sucrose is not since it does not have a free anomeric -OH.
Benedicts solution reacts with reducing sugars to oxidise them.
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