Gas Chromatography and Thin-Layer Chromatography Recitation Worksheet Name Secti

Question

Gas Chromatography and Thin-Layer Chromatography Recitation Worksheet Name Section Recitation problems may be collected randomly for a grade. 1. Four analytical/spectroscopic tools are introduced in C343 lab. For each instrument below, describe the importance of each method: How are cach of these analytical tools of chemicals (GC) Infrared (IR) Nuclear Magnetic Resonance (NMR) Thin-Layer chromatograph 2. (a)is used to separate volatile components of a mixture. A small amount, in (b) , of the sample to be analyzed is drawn up into a syringe. The syringe needle is placed into a hot injector port of the gas chromatograph, and the sample is injected. The injector is set to a temperature than the components' boiling points. So, components of the into the (e) inside the injector A carrien mixture (d) gas, such as (flows through the injector and pushes the gaseous components of the sample onto the GC column. It is within the column that separation of the components takes place. Molecules partition between the (g) stationary phase) within the GC column. After components of the mixture move through the GC column, they reach a (Ideally components of the mixture will reach the detector at differences in the partitioning between mobile and stationary phases. The detector sends a signal to the chart recorder, which results in a peak on the chart paper. The (k) molecules generating the signal, The (l) -of peaks is (the mobile phase) and (h) (the due to of the peak is proportional to the number of to the number of compounds that are in the mixture 4. During the GC analysis of banana oil product, you observe three peaks: a large peak at 1.3 min (102 cm2 and two peaks past 6 min (74 cm2 and 14 cm', respectively). Identify the compounds for these peaks. Did you achieve the desired purity of banana oil? If not, estimate the % purity of your collected product.

Explanation / Answer

1)

Gas Chromatography: This is a technique of separating different chemical compounds based on their volatility using gas as a mobile phase. Gas chromatography (GC) is commonly used for these purposes:

i) To evaluate the components of the sample which means when we have a known mixed sample, the components can be evaluated quantitatively using GC

ii) It is used as the indicator of purity of sample

iii) Used for identification of unknown compounds

Infrared spectroscopy: This is an excellent tool to identify the functional groups present in the compound. It is used to confirm the presence or absence of a particular functional group by looking at IR spectra.

Nuclear magnetic resonance (NMR) Spectrometer: It is one of the most important tools for chemists who perform synthesis in organic chemistry. From this method, we can obtain information like relative positions and numbers of spin-active nuclei like protons in a compound and identify the structure of the compound. It is also used as an analytical tool to find out product ratios and purity.

Thin-Layer chromatography( TLC): This the preliminary test for the evaluation of reaction mixtures. It depends on the polarity of the molecules. It does not provide quantitative information but comes in handy to test the purity and evaluating the presence of a certain compound.

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