8- You use gel electrophoresis to separate DNA fragments based on size. Which of

Question

8- You use gel electrophoresis to separate DNA fragments based on size. Which of the following statements are true? (Check all that apply)

A) DNA moves towards the positive electrode.

B) The smallest DNA fragments will be at the bottom of the gel and the largest DNA fragments will be at the top of the gel.

C) The largest DNA fragments will be at the bottom of the gel and the smallest DNA fragments will be at the top of the gel.

D) You should wear gloves when handling gels stained with ethidium bromide (to visualize the DNA under UV light) because ethidium bromide is a mutagen.

E) DNA moves towards the negative electrode.

A) DNA moves towards the positive electrode.

B) The smallest DNA fragments will be at the bottom of the gel and the largest DNA fragments will be at the top of the gel.

C) The largest DNA fragments will be at the bottom of the gel and the smallest DNA fragments will be at the top of the gel.

D) You should wear gloves when handling gels stained with ethidium bromide (to visualize the DNA under UV light) because ethidium bromide is a mutagen.

E) DNA moves towards the negative electrode.

Explanation / Answer

a,b and d are correct

DNA is negatively charged so it moves towards positive electrode

in this method DNA fragments are seprated according to their length and smaller fragments move faster so found at the bottom

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