3\'CGACTGACTCGACGGCCTAGCTATAG. The primer is GCTGACTG. The number of DNA fragmen

Question

3'CGACTGACTCGACGGCCTAGCTATAG. The primer is GCTGACTG. The number of DNA fragments resulting from the ddCTP tube is expected to be: For the sequencing result shown below, choose the correct sequence of template DNA 5' to 3'. Hybridization was used to determine the genotypes of 3 female family members with regard to hemophilia. The results are shown below. Each pair of dots represents the result from one person. The upper row represents hybridization using the probe designed to the mutant sequence and the lower row represents the results of hybridization using the probe designed to the wild-type sequence. What are the genotypes of the 3 individuals (1 to 3), respectively? Which of the following is not true of heterozygosity for a gene deletion? Robertsonian translocations arise from:

Explanation / Answer

92) The correct pair of sequence (starting from the lowest band then proceeding to the highest) would be -5' CTAAGA 3'.

93) First one is the homozygous mutant as it bind to the mutant probe, likewise second one is heterozygous since it bind with both probe and the third one is homozygous wild-type. Hence, the answer is (d).

94) I think- none of the above. Because all are right regarding heterozygosity for a gene deletion.

95) Robertsonian translocation is a type of reciprocal translocation which involve gene rearrangement known tp occur in five acrocentric chromosome pairs- 13, 14, 15, 21 and 22.

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