Glutamic acid has a pka values 2.2,4.3,9.7 calculate the idoelecttic point for g

Question

Glutamic acid has a pka values 2.2,4.3,9.7 calculate the idoelecttic point for glumatic acid
Name Chem 3313 Sept. 22, 2016 BioChemistry Exam 1 R-8.314 J/molK -1.987 cal/molk log (Y/1-Y) -or- log0)- -nulogKs+ nulog[L] Multiple Choice -3 points each pH-pK, + log [cbjTcal Hill equation Reaction of the peptide, ala-met-lys-ser, with phenylisothiocyanate (PITC) at pH 8.0 followed by mild acidification (first cycle of Edman method) would release: a. the labeled peptide ala-met-lys-ser-PTH. b. PTH-ala, PTH-ser, PTH-lys and PTH-met. PTH-ser and the peptide ala-met-lys. d. PTH-ala and the peptide met-lys-ser. c. e. All of the above. 2- 2+4.3 C 2. Glutamic acid has pK, values of 2.2,4.3 and 9.7. Calculate the isoelectric point for2 a. (3.25 d3. At which of the following pHvalues would histidine (pK, values of 1.8, 6.0 and 9.2) b. 4.3 c. 5.4 d. 7.0 e. 8.6 PHE be found with a net negative charge? .40, tap c. a G(1.0) a. 1.0 8.0 e. none of the above 4. In the following peptide, which amino acid is the N-terminus? Phe-Ala-Gly-Argl N A) Ala B) Phe C) Phe and Arg D) Arg E) None of the above. 5. If0.1 moles of Na:HPOs and 0.1 moles of NaH:POs are mixed in water, what is the . resulting pH? The pK, values for phosphoric acid are 2.1, 7.2, 12.4 e. 12.4 a. 2.1 b. 4.65 c. 7.2 d. 9.8 6. What pairs of atoms are involved in hydrogen bonds? D) All of the above. 7. A technique that can be used to study three-dimensional protein structure of small b) N-H and S-H c) O-H and P O a) N-H and O-H E) None of the above. proteins is A) NMR spectroscopy B) fluorescent microscopy C) paper electrophoresis E) ion-exchange chromatography D) Western blotting Which of the following amino acids is primarily responsible for this light absorption? a) phenylalanine b) histidine c) tryptophan d) proline 8. Many proteins absorb light at a wavelength of 280 nm.

Explanation / Answer

1. Option d is correct.

2. Glutamic acid Isoelectric point = ( pka1+ pka2)/2

= (2.2+4.3)/2 = 3.25

Option a is correct.

3. Option d is correct.

4. The, option b is correct.

5. 7.2, option c is correct

6. Option d is correct.

7.

8. Tryptophan, option c is correct.

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