2) Restriction enzymes used in cloning human genes into bacterial plasmids A) on

Question

2) Restriction enzymes used in cloning human genes into bacterial plasmids

A) only cuts non-plasmid DNA

B) can leave overhangs in DNA that allow complementary base pairing

C) form covalent bonds between complementary bases

D) usually cut in the ampicillin gene in the plasmid

E) are activated by cooling

4. A sequence of deoxyribonucleotides on a chromosome that encodes either a

protein, tRNA molecule, or rRNA molecule is referred to as:

A) mRNA

B) The genotype

C) a gene

D) None of these

E) the genome

Explanation / Answer

2-

B) can leave overhangs in DNA that allow complementary base pairing (this may lead to self-annealing or we can add an insert having similar overhangs usually treated by the same restriction endonuclease).

4-

C) a gene (deoxyribonucleotides- hence DNA; a gene encodes for proteins as well as rRNA & tRNA).

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