The table below shows the data for purification of a hypothetical protein. Compl

Question

The table below shows the data for purification of a hypothetical protein. Complete the table by filling in the missing values for the specific activity. Step Total Protein Total Adtivity Specifie Adtivity Yeld Punfication (mg) 17100 4840 (units) 159000 144000 , (units mg-1) 9.30 29.8 (96) 100 90.5 level Homogenization Salt fractionation 3.20 Number lon-exchange chromatography 121000 m76.1 1370 9.50 Number Gel-filtration chromatography 81600 In 69.9 51.3 126 Number Affinity chromatography 5.80 * 10In 4.57 36.5 1360

Explanation / Answer

Specific activity = total activity/ total protein

Ion exchange chromatography -

specific activity = 121000/ 1370

= 88.32 units/mg

Specific activity = 81600/69.9

= 1172.4units/mg

Specific activity = 1.269 ×104

Related Questions

Navigate

• Browse (All)
• Browse T
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.