THE EXPERIMENT: INVERSION OF SUCROSE. PROCEDURE: - Prepare solutions: o 20 g suc
ID: 715569 • Letter: T
Question
THE EXPERIMENT: INVERSION OF SUCROSE.
PROCEDURE:
- Prepare solutions:
o 20 g sucrose in 100 mL
o 100 mL of 4 N hydrochloric acid (HCl)
o 100 mL of 4 N monochloroacetic acid (MCA)
- Prepare polarimeter tubes with 50/50 sucrose solution/acid solution and take the first measurement as quickly as possible
o Prepare and measure the HCl tube first
- HCl measurements are taken every 10 minutes for the first hour and can be taken less frequently afterward. MCA measurements can be taken less frequently at convenient intervals.
- Measurements should be taken and recorded for both solutions for at least 3 hours
- Transfer solutions to separate sealed and labeled containers and let stand until the next lab period
- Final readings () for each solution are taken the next lab period
Question is Why is it important to prepare the polarimeter tubes and take the initial reading as quickly as possible?
Explanation / Answer
sucrose convert to glucose and fructose. the sucrose is dextrorotatory ( rotating right hand side ) and glucose and fructose is slightly levorotratory ( rotate left side). the progres of reaction is determine by the polary meter.
The reaction is slow in pure water, but in acidic solution the reaction is fast. therefore, it extremily advisable that once you prepare the solution take the reading as soon as possible, otherwise the sucrose will break to the fructose and glucose.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.