An Hfr strain (arg + , bio + , leu + ) is mated to an F- strain (arg - , bio - ,

Question

An Hfr strain (arg+, bio+, leu+) is mated to an F- strain (arg-, bio-, leu-). Assume that this Hfr strain transfers arg+ last. You grow the exconjugants on arg- media, supplemented with leu+ and bio+. You recover 423 arg+ exconjugants. These are tested individually for bio and leu and you find the following distribution of genotypes:

                                    arg+, bio+, leu+           360

                                    arg+, bio+, leu-            9

                                    arg+, bio-, leu+            0

                                    arg+, bio-, leu-             54

Determine the gene order and the recombination distances between the three genes.

Explanation / Answer

gene order is arg bio leu

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