7) Identify one of the unknown proteins by following the instructions on the acc

Question

7) Identify one of the unknown proteins by following the instructions on the accompanying handout and answer the que stions below about your protein. Last names starting with A-H please work with unknown 1; I-R unknown 2 and S-Z unknown 3. (10 pts) me Unknown Peptide 1 Peptide 2 The following questions pertain to your unknown protein that you should have identfied by now Please turn in the NCBl entry on your protein (obtained in step 13 of the exercise) stapled to your problem set Most of the answers to the questions below can be found on that print out if you look carefully a) What is your unknown number? b) What is the name of your unknown protein? c) Why are there multiple hits for what appears to be the same enzyme? d) Can you positively identify the organism from which this enzyme derived? If so, what organism is it from? Ifnot, why not? e) What is its Enzyme Classification number 0) What is the Swiss- Prot/UniPROT accession number for this protein? Page S of 8 Feig - CHM5600 Problem Set 1 Fal 2018 Due 9V18/18 g) When you entered yourpeptde in backwards, did you get a significant match to anything in the database? What does this teach you about the importance of directionality and orientation in biobgical macromolecules? h) Find the peptides you used to identify yourunknown protein within the full protein sequence. Highlight the peptide fragments. Are the sequences contiguous to one another and does it matter? Please explain. i) Compare the results from the two searches that used both peptide fragments (Searches 3 and 4). Did you get identical results? This comparison calls into play gaps and gap penaltes, but the program has a maximum gap size of 40 amino acids. Explain yourresults from Searches 3 and 4 in relation to gaps and gap penalties.

Explanation / Answer

THE UNKNOWN PROTIEN

1) Cytidine Deaminase (CDA)

it a protein coding gene which isfound in human beings

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