If the Tn10 transposon (carrying the tet gene) is used to mutagenize a bacterium

Question

If the Tn10 transposon (carrying the tet gene) is used to mutagenize a bacterium for the purpose of creating a mutant in the His operon what would be the most appropriate selection media? Rich media that contains all of the amino acids supplemented with tetracycline, Minimal media just supplemented with His Minimal media supplemented with tetracycline and His Minimal media supplemented with tetracycline alone A strain of E. coli is trp his lac . Which medium would this bacterium grow on? lactose+ histidine + tryptophan glucose + histidine + tryptophan minimal glucose, no histidine, no tryptophan lactose, no histidine, no tryptophan The episode in an Hfr strain is inserted near the trp (tryptophan) locus. This Hfr strain is incubated with an F trp strain. We isolate a trp' strain that can readily donate the trp' phenotype to other F trp strains. No other traits are transferred. What has happened? An F^+trp plasmid has been generated Typical Hfr mating Transduction occurs Transformation occurs What characterises make bacteria like E. coli attractive as model organisms Short generation time, relatively simple genome, easily mutagenized, haploid, gene mechanisms identical to eukaryotes Short generation time, relatively simple genome, easily mutagenized, haploid. Short generation time, relatively complex genome, easily mutagenized, haploid Short generation time, realtively simple genome, easily mutagenized, diploid.

Explanation / Answer

Q 1

c. Minimal media supplemented with tetracycline and His

Q 2

a. Lactose + histidine + tryptophan

Q 3

a. An F+ trp plasmid has been generated

Q 4

Short generation time, relatively simple genome, easily mutagenized, gene control mechanisms identical to eukaryotes.

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