I have a data analysis immunobiology question I need some help with: Here is som

Question

I have a data analysis immunobiology question I need some help with:

Here is some background information:

When C57BL mice are immunized with the haptennitrophenacetyl (NP) on a suitable carrier, they makea robust response, with germinal centers waxing andwaning over a 21-day period as antigen is cleared. This is a nice model because (1) the response iscomprised mainly of B cells expressing a VH genecalled 81X paired with a lambda light chain; and (2)in secondary responses, the amino acid at position94 in the heavy chain has been replaced by atyrosine, lending higher affinity than the unmutatedheavy chain. Thus, by using FACS with acombination of reagents to track memory B cellmarkers, NP-binding, and lambda light chainexpression, one can detect and sort memory cellpopulations, then perform single cell PCR to analyzeV-region sequences. In an effort to understand thegeneration and dynamics of memory B cells, aninvestigator immunized C57BL mice once with NP. He then determined the total number of memory Bcells in the spleen and the proportion of memorycells with the tyr-94 mutation at varying times afterimmunization.

The data for this experiment is below:

Here is the question I need some help with:

1a. What do you conclude from the upper graph?

1b. What do you conclude from the lower graph?

Explanation / Answer

The upper graph indicates that the number of NP-binding memory B cells increase very rapidly initially and then decrease to some limit to get stabilized. The lower graph indicates that the memory cells are growing gradually at some pace and then getting stabilized for certain period. Again they grow in 4 to 6 weeks period and again stop to maintain a stable population.

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