Question: Why do you think it is necessary to use a thermostable form of DNA pol

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Question: Why do you think it is necessary to use a thermostable form of DNA polymerase (e.g. Taq polymerase) used in PCR? Is it necessary to use a thermostable form of DNA polymerase in the techniques of dideoxy DNA sequencing or site-directed mutagenesis?

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Explanation / Answer

during the PCR WORKING, THERE IS ANNEALING AND DENATURATION OF DNA AS TEMPERATURE RISES UPTO 90 DEGREE denaturation of DNA WHICH REQUIRE THERMOSTABLE TAQ POLYMERASE

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