A stock solution was prepared by dissolving and diluting 0.725g of glucose (MM=1

Question

A stock solution was prepared by dissolving and diluting 0.725g of glucose (MM=180.156g/mol) to 100.00mL with deionized water.

A dilution was prepared from the stock solution by treating 15.00mL of the stock solution with o-toluidine and diluting to 100.00mL with deionized water. Colorimetric determination gave that the transmittance of the sample was 55.2% when the path length of the light through the solution was exactly 1cm.

Calculate the Absorbance for the species.

PLEASE SHOW STEP BY STEP INSTRUCTION

Explanation / Answer

Transmitance% =55.2 , hence T=55.2/100 =0.55 A=-logT = -log(0.55) = 0.2596

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