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Using this graph estimate the absorbance expected for a solution with a concentr

ID: 729624 • Letter: U

Question

Using this graph estimate the absorbance expected for a solution with a concentration of 2.20 times 10-5 M in Allura Red (to two significant figures) Using this graph, determine the concentration of a solution with an absorbance of 0.220 ABS (to two significant figures)? If the unknown solution needed to be diluted by adding 9 mls of water to 1 ml of the unknown in order for its absorbance to fall within the abs range of the calibration curve, and the absorbance of the diluted solution was 0.5 What was the concentration of the diluted solution? What was the concentration of the original unknown solution?

Explanation / Answer

C of diluted solution = 1.5/0.8 = 1.875 x 10^-5 M C of original unknown solution = 10 x 1.875 x 10^-5 = 1.875 x 10^-4 M