If one sought to create a viable population of cells in a culture in which each

Question

If one sought to create a viable population of cells in a culture in which each of the cells were in the same cell cyle phase or in cell synchrony, then one would do all of the following except:

a. Treat a culture with colcemid, wait a period of time, then wash the colcemid out

b. Add colchicine to a culture, wait a period of time, then wash the colchicine out

c. Adherent cells in the mitotic phase begin rounding and hence can detach and be collected through shaking the petri dish in which the cells have been cultured.

d. Add a microtubule-depolymerizing drug like vinblastine, wait a period of time and then wash the vinblastine out of the culture

e. All of the above are correct

Explanation / Answer

The correct answer is option (C).

C) Adherent cells in the mitotic phase begin rounding and hence can detach and be collected through shaking the petri dish in which the cells have been cultured.

These cells are attached firmly during the mitosis phase of cell cycle.

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