You are analyzing an amino acid and make the following observations: 1. Amino ac

Question

You are analyzing an amino acid and make the following observations:
1. Amino acid composition is (A, S, K, F, V, P, G, R, L, T, Q, M)

2. Edman degradation gives the following products for the first five cycles:
1. Pro, Gly 2. Ser, Ala 3. Arg, something weird 4. Phe 5. Leu

3. Chymotrypsin digestion gives a fragment LMTQ and one other product

4. Trypsin digestion gives GSR and one other product

What is the original peptide? (Hint: lysine has an ?-amino group which is forming a peptide bond with another amino acid, and is therefore trypsin-resistant).

Explanation / Answer

3

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