The following table shows several bacterial strain lac operon genotypes (some ar
ID: 7445 • Letter: T
Question
The following table shows several bacterial strain lac operon genotypes (some are partial diploids).a) Fill in the blanks in the “lactose absent” and “lactose present” columns in this table. (+) means significant levels of active ß-galactosidase enzyme can be detected. (–) means no significant levels of active ß-galactosidase enzyme. The first line is filled in for reference.
Strain genotype
Lactose absent
Lactose present
1. lacI+ lacP+ lacO+ lacZ+ lacY+
2. lacI+ lacP+ lacOc lacZ+ lacY+
3. lacI+ lacP+ lacOc lacZ– lacY+
4. lacIs lacP+ lacOc lacZ+ lacY+
5. lacI– lacP+ lacO+ lacZ+ lacY+ / lacI+
6. lacIs lacP+ lacO+ lacZ+ lacY+/ lac I+
b) If lactose is absent and glucose is present in the culture medium of strain 4, will significant levels of active ß-galactosidase enzyme be detected? Explain… in brief, just because there is lots of room left, you don’t need to fill it.
Explanation / Answer
Lactose absent
Lactose present
1. lacI+ lacP+ lacO+ lacZ+ lacY+
-----------
------
2. lacI+ lacP+ lacOc lacZ+ lacY+
--------------
+
3. lacI+ lacP+ lacOc lacZ– lacY+
---------------
----------
4. lacIs lacP+ lacOc lacZ+ lacY+
----------------
-----------
5. lacI– lacP+ lacO+ lacZ+ lacY+ / lacI+
----------------
+
6. lacIs lacP+ lacO+ lacZ+ lacY+/ lac I+
--------------
---------
Lactose absent
Lactose present
1. lacI+ lacP+ lacO+ lacZ+ lacY+
-----------
------
2. lacI+ lacP+ lacOc lacZ+ lacY+
--------------
+
3. lacI+ lacP+ lacOc lacZ– lacY+
---------------
----------
4. lacIs lacP+ lacOc lacZ+ lacY+
----------------
-----------
5. lacI– lacP+ lacO+ lacZ+ lacY+ / lacI+
----------------
+
6. lacIs lacP+ lacO+ lacZ+ lacY+/ lac I+
--------------
---------
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