Addition of [____] from human bladder carcinoma to a culture of mouse 3T3 cells

Question

Addition of [____] from human bladder carcinoma to a culture of mouse 3T3 cells causes a rare transformation in which a focus of cells begins to divide abnormally. To clone the [____] responsible for the transformation, advantage is taken of the fact that most human genes have nearby Alu repetitive sequences. Total DNA from the initial focus of transformed mouse cells is isolated, and the [____] is separated from adventitious, contaminating human DNA by secondary transfer to mouse cells for formation of another focus of transformed cells. The total DNA from this secondary transformation is cloned into bacteriophage lambda. Almost all of these bacteriophage will contain mouse DNA, but an occasional phage will contain human DNA, which also contains the [____], as identified by hybridization on a filter paper with a(n) [____] probe.

(1) cells; defective proto-oncogene; transforming DNA; proto-oncogene; oncogene

(2) oncogenic DNA; oncogene; oncogene; oncogene; proto-oncogene

(3) transforming DNA; mutant tumor suppressor; mouse DNA; tumor suppressor; Alu sequence

(4) retroviruses; retroviral sequence; retrovirus; Src sequence; Src

(5) DNA; oncogene; transforming DNA; oncogene sequence; Alu sequence

Explanation / Answer

(5) DNA; oncogene; transforming DNA; oncogene sequence; Alu sequence

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