You are planning a transformation experiment and deciding how to do the transfor

Question

You are planning a transformation experiment and deciding how to do the transformation and how to select for transformed bacteria. The best plan is to:

A) extract DNA from an auxotroph and add it to prototrophic cells and grow on minimal media

B) extract DNA from arg- cells and add it to arg+ cells and grow on minimal media

C) extract DNA from arg+ cells and add it to arg- cells and grow on minimal media

D) extract DNA from StrS cells and add it to StrR cells and grow on media with Streptomycin

E) All of the above are equally good strategies

Explanation / Answer

ans-The best plan will be to extract DNA from StrS cells and add itto StrR cells and grow on media with strptomycin.

Reason: The transformed cell willhave some effect to antibiotics and the colonies will differ in antibiotic containing media as compared to non transformed or original wildtype bacteria.To identify the transformation this is the best practice.

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