To test if the OG can change a normal cell, you clone the ORF this gene into an

Question

To test if the OG can change a normal cell, you clone the ORF this gene into an expression vector and transfect it into sea urchin embryos right after they are fertilized. You then grow the embryos for the times indicated below (from 26 to 146 hours post-fertilization), and you perform a western analysis on the lysates of the embryo using an anti-Cyclin D antibody. Your results are shown below: Top figure: un-transfected sea-urchin embryos; bottom figure: sea urchin embryos transfected with your oncogene. Based on these results, what has the oncogene done to the embryos? Please explain briefly. If the oncogene was stably transfected, and it was integrated into the genome at a random site, would these results suggest the oncogene is dominant or recessive in its actions? Please explain briefly.

Explanation / Answer

a. Oncogene will change the normal pattern of cell growth into a cancerous cell as seen by the cyclin-D western blot analysis, where cyclin D expression is required for G1 to S phase transition.

b. The oncogene is dominant in nature as shown in western blot. Increased expression can be seen at a regular pattern of time frame. After this, there is a reduction in expression which might be because the gene is expressed only when required.

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