Help!. Put the following steps for the mechanism of chymotrypsin catalysis in or

Question

Help!. Put the following steps for the mechanism of chymotrypsin catalysis in order. 1. His 57 catalyzes removal of H from Ser 195 2. His 57 donates H to N of sissile peptide bond, tetrahedral intermediate decomposes.3 3. Water donates H to his 57. 4. Resulting OH attacks carbonyl of remaining substrate. 5. The portion (C-terminal end) of original substrate with the new carboxylate terminus diffuses away. 6. His 57 donates H to Ser 195 O, leading to collapse of tetrahedral intermediate. 7.. the portion (N-terminal end) of original substrate with the new carboxylate terminus difuses away 8. Ser 195's nucleophilic O attacks carbonyl C of substrate

Explanation / Answer

The order goes: First --> Last 1. His 57 catalyzes removal of H from Ser 195 hydroxyl. 2. Ser 195's nucleophilic O attacks carbonyl C of substrate. 3. His 57 donates H to N of sissile peptide bond, tetrahedral intermediate decomposes. 4. The portion (C-terminal end) of original substrate with the new carboxylate terminus diffuses away. 5. Water donates H to his 5. 6. Resulting OH attacks carbonyl of remaining substrate. 7. His 57 donates H to Ser 195 O, leading to collapse of tetrahedral intermediate. 8. The portion (N-terminal end) of original substrate with the new carboxylate terminus difuses away 8. Ser 195's nucleophilic O attacks carbonyl C of substrate.

Related Questions

Navigate

• Browse (All)
• Browse H
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.