In the Yeast Two-Hybrid Screen technique, suppose I use a transcription factor i

Question

 In the Yeast Two-Hybrid Screen technique, suppose I use a transcription factor in which the protein of the Activation Domain (AD) always binds the protein of the DNA Binding Domain (BD). What will happen in my Yeast Two-Hybrid experiments when I test for an interaction between Protein A and Protein B by fusing them to the AD and BD, respectively?

A. The outcome of the test will depend upon whether Protein A and Protein B interact.
B. You will always observe a negative test that suggests no interaction between Protein A and Protein B.
C. You will always observe a positive test that suggests an interaction between Protein A and Protein B.
D. All the yeast cells will die
E. B and D

Explanation / Answer

E. B and D

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