Triose phosphate isomerase catalyzes the conversion of glyceraldehyde-3- phospha

Question

Triose phosphate isomerase catalyzes the conversion of glyceraldehyde-3- phosphate to dihydroxyacetone phosphate. Glyceraldehyde-3-P dihydroxyacetone-P The Km of this enzyme for its substrate glyceraldehyde-3-phosphate is 1.8 x 10- 5M. When [Glyceraldehyde-3-P] = 30?M, the rate if the reaction, v, was 82.5?Msec-1. a. What is Vmax for this enzyme? b. Assuming that3 nanomoles/mL (ET) of enzyme was used in this experiment, what is the kcat for this enzyme? c. What is the catalytic efficiency (kcat/Km) for triose phosphate isomerase?

Explanation / Answer

a) given v= 82.5 uM/s , [S] = 30 uM , Km = 1.8 x10^ -5 = 18 uM , v = Vmax[S]/(Km+[S]) , 82.5 = Vmax(30)/( 18 + 30) , Vmax = 132 uM/s, b) Vmax = kcat [Eo] , given [Eo] = 3nM = 0.003 uM, k cat = ( 132/0.003) = 44000 s-1, c) catalytic efficiency = kcat/ Km = 44000/1.8 x10^ -5 = 2.44 x10^ 9 M/s

Related Questions

Navigate

• Browse (All)
• Browse T
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.