6. An enzyme-catalyzed reaction produces a product that has a maximum absorbance

Question

6. An enzyme-catalyzed reaction produces a product that has a maximum absorbance at 412nm. The extinction coefficient is 4000M^-1cm^-1, A l0 to-l dilution of the enzyme is made and 20 u.L of the dilution are put into a cuvette with 80 ul of water and l.9mL of reaction cocktail. The absorbance change per minute is 0.05.

a. How many units are in the cuvette if 1 U = 1 umol/min?

b. How many units are in the cuvette if 1 U = nmol/min?

c. What is the relative activity of the undiluted enzyme (1U =1 umol/min)?

Explanation / Answer

ans a.) 48 uL

b.) 64ul

c.) 1.2 ml

for solution rate the ans.

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