Which of the following globular (or ellipsoid) proteins would elute first (in th

Question

Which of the following globular (or ellipsoid) proteins would elute first (in the void volume, Vo) from the following gel-filtration columns? If there is more than one answer, select all that apply.

Bio-Gel P-30 exclusion limit of 40 kDa
a.) %u03B1-amylase (54,000 Da)
b.) %u03B2-amylase (210,000 Da)
c.) lysozyme (15,000 Da)
d.) lingual lipase (52,000 Da)
e.) standard (29,000 Da)
f.) lactoferrin (75,000 Da)

Bio-Gel P-60 exclusion limit of 60 kDa
a.) %u03B1-amylase (54,000 Da)
b.) %u03B2-amylase (210,000 Da)
c.) lysozyme (15,000 Da)
d.) lingual lipase (52,000 Da)
e.) standard (29,000 Da)
f.) lactoferrin (75,000 Da)

Bio-Gel P-100 exclusion limit of 100 kDa
a.) %u03B1-amylase (54,000 Da)
b.) %u03B2-amylase (210,000 Da)
c.) lysozyme (15,000 Da)
d.) lingual lipase (52,000 Da)
e.) standard (29,000 Da)
f.) lactoferrin (75,000 Da)
Which of the following globular (or ellipsoid) proteins would elute first (in the void volume, Vo) from the following gel-filtration columns? If there is more than one answer, select all that apply.

Bio-Gel P-30 exclusion limit of 40 kDa
a.) %u03B1-amylase (54,000 Da)
b.) %u03B2-amylase (210,000 Da)
c.) lysozyme (15,000 Da)
d.) lingual lipase (52,000 Da)
e.) standard (29,000 Da)
f.) lactoferrin (75,000 Da)

Bio-Gel P-60 exclusion limit of 60 kDa
a.) %u03B1-amylase (54,000 Da)
b.) %u03B2-amylase (210,000 Da)
c.) lysozyme (15,000 Da)
d.) lingual lipase (52,000 Da)
e.) standard (29,000 Da)
f.) lactoferrin (75,000 Da)

Bio-Gel P-100 exclusion limit of 100 kDa
a.) %u03B1-amylase (54,000 Da)
b.) %u03B2-amylase (210,000 Da)
c.) lysozyme (15,000 Da)
d.) lingual lipase (52,000 Da)
e.) standard (29,000 Da)
f.) lactoferrin (75,000 Da)
Which of the following globular (or ellipsoid) proteins would elute first (in the void volume, Vo) from the following gel-filtration columns? If there is more than one answer, select all that apply.

Bio-Gel P-30 exclusion limit of 40 kDa
a.) %u03B1-amylase (54,000 Da)
b.) %u03B2-amylase (210,000 Da)
c.) lysozyme (15,000 Da)
d.) lingual lipase (52,000 Da)
e.) standard (29,000 Da)
f.) lactoferrin (75,000 Da)

Bio-Gel P-60 exclusion limit of 60 kDa
a.) %u03B1-amylase (54,000 Da)
b.) %u03B2-amylase (210,000 Da)
c.) lysozyme (15,000 Da)
d.) lingual lipase (52,000 Da)
e.) standard (29,000 Da)
f.) lactoferrin (75,000 Da)

Bio-Gel P-100 exclusion limit of 100 kDa
a.) %u03B1-amylase (54,000 Da)
b.) %u03B2-amylase (210,000 Da)
c.) lysozyme (15,000 Da)
d.) lingual lipase (52,000 Da)
e.) standard (29,000 Da)
f.) lactoferrin (75,000 Da)

Explanation / Answer

P-30 = A,b,d,f

p-60 = b,f

P-100 = B

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