If a mixture of lysine and aspartic acid is applied to an anion exchange column
ID: 78964 • Letter: I
Question
If a mixture of lysine and aspartic acid is applied to an anion exchange column ( + charged beads) at pH 7, the first amino acid to elute would be _____________. __________ is an example of a posttranslationally modified amino acid. The organic portion of the heme group in hemoglobin is the ________. In sickle-cell adult hemoglobin, HbS, the beta 6 position is a Val which is a _________ amino acid, while in normal adult hemoglobin (HbA), it is a Glu which is a __________ amino acid. (Name a property of each residue which explains the difference in the resulting hemoglobin molecule). The PI of sickle cell hemoglobin would be _____________ then that of normal adult hemoglobin. In eukaryotic genes the noncoding regions are called _____________ During translation, the cod on for the amino acid ___________ serves as the start signal. (Name the amino acid in either prokaryotes or eukaryotes for credit). Amyloid protofibrils contain ordered arrays of ____________ secondary structure The absorbance of a protein solution was measured be 5.6 at 280 nm. The extinction coefficient (epsilon) for the protein .70 cm^2 mg^-1 and the absorbance was measured in a with a pathlength of 0.65 cm. What is the concentration of the protein? ________________. Scurvy is due to _________ which causes weakening of collagen fibers due to failure to hydroxylate the sidechains of _________ residues.Explanation / Answer
Answer 1: aspartic acid (As explained below)
Aspartic acid would elute first because it is the less positive of the two.
Answer 2: phosphorylation of glycogen phosphorylase (As explained below)
Phosphorylation of glycogen phosphorylase is a common post-translational modification that regulates the activity of glycogen phosphorylase.
Answer 3: protoporphyrin (As explained below)
As per morphology, heme comprises of organic component, protoporphyrin and iron.
Answer 4: non-polar, polar (As explained below)
Virtue of Valine in causing sickle celled anemia is due to its being non-polar and being able to replace the polar amino acid glutamic acid in normal b-globin chains.
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