The hydrolysis of sucrose to glucose and fructose is catalyzed by an enzyme call
ID: 789686 • Letter: T
Question
The hydrolysis of sucrose to glucose and fructose is catalyzed by an enzyme call invertase.
The hydrolysis of sucrose to glucose and fructose is catalyzed by an enzyme called invertase. Use the data to the right to determine the value of Rmax (the maximum rate of the enzyme, often called maximum velocity, Vmax) and Km, the Michaelis-Menten constant. You can use the Lineweaver-Burk equation to determine the kinetic constants for this enzyme, but you must first convert the data to a usable format. Enter the graphable form of the data below. If you would use the original data, enter the original value in the corresponding blank. Give all values in units that include mmol.Explanation / Answer
a.) convert [s] to mmol then divide 1 by the value (recipricol) -- for R just divide 1 by the value given
3.57--> .0280
2.22--> .00450
7.04--> .142
13.0-->.0769
b.) rise over run - difference between y values (R) over difference between x values (s) and then just plug one set of points into y=mx+b to get b
m=2.77
b=.0644
c.) Rmax = 1/y-inter and slope=Km/Rmax
Rmax=15.5
Km= 42.9
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