Write all the possible mRNA sequences that can code for the simple tripeptide se

Question

Write all the possible mRNA sequences that can code for the simple tripeptide segment: Trp-Val-His b) Many techniques in biochemistry and molecular biology require you to create a "probe" for specific sequences. Suggest nucleic acid probes that could be used to fish out the mRNA for the sequence above. c) For part b) you should have obtained multiple probes. Let's say you want to detect the mRNA from a particular protein but you also want to minimize the number of probes that you need to make. What would you look for in the protein?

Explanation / Answer

Answer:

4. a. The possible mRNA sequence is:

5' ugggugcau 3'

b. Probes are fragments of DNA or RNA of variable length that can be used in DNA or RNA samples to detect the presence of nucleotide sequences that are complementary to the sequence in the probe.

Nucleic acid probes that can be used are: (can be of varying length)

i. 5' atgcaccca 3'

ii. 5' caccca 3'

c. Specific unique sequences in the protein sequence should be looked for inorder to design highly specific oligonucleotide probes such that they can be specifically used to distinguish between members of the same gene family.

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