8. you have sequence a short piece of DNA and produced the gel shown in figure Q

Question

8. you have sequence a short piece of DNA and produced the gel shown in figure Q 10 - 40.

A. What is the sequence of the DNA, starting from the 5' end?

B. If you knew that this sequence is from the middle of a protein coding cDNA clone, what amino acid can be deduced from this sequence?

Could I get some help with this problem.

8 you have sequenced a short piece of DNA and produced the gel shown in FigureQ1040 (a) What is the sequence of the DNA, starting from the 5 end? (b) If you knew that this sequence is from the middle of a preten- coding cDNA clone, amina acid sequence can be deduced from this what sequence?

Explanation / Answer

The sequence from 5'end is

TAG ACT GAC CTG

and the amino acid it is associated with is as follows:

TAG-stop codon

ACT codes for Threonine

GAC codes for Aspartic acid

CTG codes for Leucine

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