your friend works at the Centers for Disease Control and has discovered brand ne
ID: 79189 • Letter: Y
Question
your friend works at the Centers for Disease Control and has discovered brand new virus that has recently been introduced into the human population. She has just developed in USA that allows her to detect the virus by using PCR products made from the blood of infected patients. The essay uses primers in the PCR reaction that hybridize to sequences in the viral genome.
your friend is distraught because of the results she obtained when she looks happy CR products made using the blood of three patients suffering from the viral disease, using her own blood and using a leaf from her petunia plant
you advise your friend not to panic as you believe she is missing an important control. Which of the choices listed below is the best control for clarifying the results of her essay explain your answer
A. a PCR reaction using blood from a patient who is newly infected but does not yet show symptoms
B. a PCR reaction using blood from a dog
C. a PCR reaction using blood from an uninfected person
D. repeating the experiment she has already done with a new tube of polymerase
could someone help me with this problem I think I got the right answer but I just want to make sure please explain
patient 1 patient 2 patient 3 self petuniaExplanation / Answer
A. a PCR reaction using blood from a patient who is newly infected but does not yet show symptoms
This can be used as a control because, the symptoms for an infection is observed only when the viral genome integrates with the DNA of the patient. In a patient who is newly infected and the symptoms are not obvious, the viral genome would be intact in the virus. While doing PCR reaction from the blood of this patient, the viral genomic DNA would form a separate band and would help in easier diagnosis of the virus.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.