your friend has isolated a protein present in the cheek cells of all straight A
ID: 79190 • Letter: Y
Question
your friend has isolated a protein present in the cheek cells of all straight A seniors at your school. She says that this protein helps you remember everything you read and therefore will help you cut down on the number of hours needed to study for exams. She sequences the protein, which she calls geniuszyme and Designs a probe to isolate the gene that encodes it. To make sure she designs the probe correctly, she consults with the company that clone Factor 8. They have 100% confidence that her probe will work she also obtained a high-quality liver cdna library from the company and uses her probe to try to isolate the gene for geniuszyme. Unfortunately she has she is unable to isolate any clones.
A. what is the likely explanation for her failure
B. not to be discouraged comma your friend has obtained some genomic DNA isolated from the nuclei of liver cells and has made a genomic library from that DNA. Do you expect you will succeed and isolating the geniuszyme from this Library? Why or why
Explanation / Answer
A. The purity and concentration of your starting materials is very imporant for successful cloning, probe is refered as sequence of DNA or RNA which hybridize with sequence of interest specifically. Thus, probe which she designed may not pair with the recognition sequence of Interest.
B. The important point to consider before we proceed for geniuszyme cloning. We construct a vector with a carrier of geniuszyme which is then allow to replicate thus making its multiple copies. In this state the newly formed cell can also be expressed in the host cell producing the product of the geniuszyme which is needed by us.
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