In an agarose gel DNA electrophoresis, which of the following DNA molecule will

Question

In an agarose gel DNA electrophoresis, which of the following DNA molecule will move faster 12,000 Kbp 10, 800 kbp 18,000 kbp 5,000 kbp In an agarose gel DNA electrophoresis, the DNA fragments are separated based on their Density Size Electrical charged. Nucleotides sequences All of the above Proteins using are usually separated using polyacrylamide gel rather than agarose because: Polyacrylamide polymerizes faster than agarose gel Polyacrylamide gel has pore size larger than the size of protein molecules Polyacrylamide gel has pore size similar to size of protein molecules None of the above Restriction endonucleases Recognize specific sequence on double stranded DNA Hydrolysis the phosphodiester bond of DNA backbone Randomly recognize and cut DNA sequences a and b b and c SDS-PAGE is used to: Detect the numbers of proteins in a sample Determine the molecular weight of proteins Compare the proteins profiles of different species All of the above In a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS -PAGE), the movement of the protein molecules depends Directly on net charge of molecules Inversely on shape of the molecules Directly on size of the molecules Inversely on size of molecules We can use agarose gel as well as polyacrylamide gel to resolve both protein and DNA False, agarose gel is only used for DNA and polyacrylamide is used for proteins True, polyacrylamide gel is used for very large protein and DNA molecules False, proteins cannot be resolved on an agarose gel True, different concentrations of agarose and de can be used to resolve both molecules based on the size of molecule of intrest When you are intended to resolve very large protein molecules on a polyacrylamide gel, you need a Low concertation gel High concentration gel Digest the protein first

Explanation / Answer

35. The answer is D. 5000 kbp. In electrophoresis the small molecules will move faster when compared with long molecules.

36. The answer is b. Size

37. The answer is b.

38. The answer is d.

39. The answer is d.

40. The answer is c.

41. The answer is a.
but for DNA sequencing we can use PAGE, but for protein we never use Agarose gel

42. The answer is a.

Related Questions

Navigate

• Browse (All)
• Browse I
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.