Yeast is cultivated in a CSTR bioreactor, glucose is employed as substrate Gluco
ID: 79719 • Letter: Y
Question
Yeast is cultivated in a CSTR bioreactor, glucose is employed as substrate Glucose concentration in feed stream is 10 g/L and the specific flow rate of feed steam is 0.06/h. While the CSTR achieved steady state, it is found that: Glucose conc. in reactor = 0.85 g/L Ethanol conc. in reactor = 3.3 g/L Glycerol conc. in reactor = 0.25 g/L Q_co2 = 9.2 mmol/L-h Assume there is no ethanol lost due to evaporation. The simplified yeast metabolic pathway is as follow: What are the rate of consumption of glucose and the rates of production of ethanol, glycerol and CO_2? According to the pathway diagram given, how many fluxes and intermediate metabolites involved in the pathway? Among those fluxes, how many are known fluxes?Explanation / Answer
(1)
Rate of consumption of glucose = 0.0047/10 = 0.0047 mmol/L
Rate of consumption of ethanol = 0.99/3.3 = 0.3 mmol/L
Rate of consumption of Co2 = 9.2/10 = 0.92 mmol/L
(2) Number of intermediate metabolites = 5
Number of known fluxes = 4
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