The following table shows where different restriction endonucleases (restriction

Question

The following table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine.

Plasmid DNA and a gene of interest are cut with the enzyme PpuMI. Write a possible seqence of bases for the sticky end of the gene in the 5' to 3' direction.

The following table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine.

Explanation / Answer

its GWC

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