Penicillase is an enzyme secreted by bacteria to inactivate the antibiotic penic

Question

Penicillase is an enzyme secreted by bacteria to inactivate the antibiotic penicillin. The enzyme has a molar mass of 30,000 and a single active site. The catalytic rate constant is 2000 s-1 and the Michaelis constant is KM = 5 times 10-5 M. In response to treatment with 5 microM of penicillin, a 1 mL (milliliter) suspension of bacterial cells secretes 0.04 micrograms of penicillase. Assuming that the enzyme quickly equilibrates with its substrate, what fraction of the enzyme will be complexed with penicillin in the early stages of the reaction?

Explanation / Answer

Penicillase is an enzyme secreted by bacteria to inactivate the antibiotic penic

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