The enzymes weaken substrate bonds chemically and/or by mechanically and that io

Question

The enzymes weaken substrate bonds chemically and/or by mechanically and that ionic cofactors may participate in substrate activation in the enzyme active site. When analyzing an enzymatic reaction, it is helpful to think in terms of the attacking group in one substrate and its target in the other substrate. For each of the following reactions, name and describe the attacking functional group, the atom which actually makes the nucleophilic attack, the target functional group, and the target atom within that group. For each reaction identify how each substrate is made more reactive and the specific means by which this is accomplished. There will be some similarities and differences in these reactions If any of the specific explanations for a given reaction are unknown, explain any possible answers which are currently under consideration. 1) DNA polymerase reaction

Explanation / Answer

In DNA polymerisation reaction the polymerase acts on the single strand of DNA and after reading help in making complementary strand with the help of oligonucleotide. The target compound is 3' OH group upon which it acts and bind nucleotides to form a new strand in 5'-3' direction.the reaction may be accelerated by providing primer and suitable oligonucleotide availablity faster.

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