Your lab mate shows you the result of her in situ hybridization hybridizing a si
ID: 80454 • Letter: Y
Question
Your lab mate shows you the result of her in situ hybridization hybridizing a single stranded RNA probe for p53 labeled with digoxigenin on normal and cancer colon tissue sections (shown above). Expression results in a bluish color stain. How would you interpret this experiment? What advice would you give your lab mate for additional or follow up experiments to do?
In situ hybridization with Dig p53 probe on normal colon section (BCIPNET, x 100) In situ hybridization with Dig p53 probe on colon cancer section. (BCIPNET, x 100)Explanation / Answer
P 53 is a tumour supressor gene.It is activated by DNA damage and increases the transcription of P21 inhibiting the activity of cyclin/CDK complex and thus preventing the cells from entering Sphase
In mutated condition , P 53 is over expressed and results in the carcinomatous changes in the cells.
To understate the metastasis of cancer the cells from different states of metastaasis has to be subjected to examination
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