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Gene drives i. What is the difference between a gene drive and daisy drive? ii.

ID: 80469 • Letter: G

Question

Gene drives

i. What is the difference between a gene drive and daisy drive?

ii. What is the goal of daisy quorum drive?

Synthetic biology

i. What are the two goals of synthetic biology

ii. How does negative and positive autoregulation affect the response time to steady state

iii. What is the effect of a coherent feedforward loop?

iv. What is the effect of an incoherent feedforward loop?

v. What is the effect of the repressilator?

vi. What is noise in biology?

vii. Name three things that have been made with synthetic biology

Explanation / Answer

Gene drive

Ans-1gene drive is insertion of a specific sequence of gene to engineer an organism. It include cas9 and guide RNA while in daisy drive cas9 is present but guide RNA is absent.

Ans-2 in daisy drive the bottom gene drive the next gene but not itself so the natural selection does not affect this. They remain safe from natural selection

Synthetic biology

Ans-1 a) designing new biological part or system

b) redesigning the existing biological part for better working

Ans-2negative autoregulation faster the reaction time by reducing its own transcription while positive autoregulation slow reaction time by enhancing its own transcription.

Ans-3 the coherent feed forward loop is responsible for sign signalling delay which means short pulse have no effect but long and persistent will have effect after a short delay

Ans-4 the incoherent feedforward loop accelerate the process. It can accelerate transcription of any gene.

Ans-5 it affect the expression of next gene by repressing the function of next gene.

Ans-6 it is the random variability between the cells. Even similar cells can show different expression and protein synthesis.

Ans-7 (a) acrylic acid

(B) 1,3 butadiene

(C) adipic acid