In the cassette shown in Figure 1a, two different lox sites are used. What seque

Question

In the cassette shown in Figure 1a, two different lox sites are used. What sequences in the lox sites must he different to prevent the two sites from recombining with each other? (d) When two or more different GIT variants are expressed in a neuron, the final color is a blend that reflects the amount of each GFP present. In mice with three cassettes, one GFP variant is expressed from each cassette (assuming that the lox sites preclude recombination between. Ten different colors are possible. Describe the combinations leading to the different colors.

Explanation / Answer

(1) The lox sites must differ in their core sequences so that lox1 reacts with lox1 only and lox2 reacts only with the lox2.

(2) Cre-mediated recombination causes different patterns of fluorescent protein expression, producing different colors in each neuron.
Lox1 x Lox1 - RFP - gives red color
Lox2 x Lox2 - YFP - gives yellow color

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