Exactly 5.00ml aliquots of a solution containg phenobarbital were measured into

Question

Exactly 5.00ml aliquots of a solution containg phenobarbital were measured into 50.00 ml volumetric flasks and made basic with KOH. The following volumes, 0.1ml,0.15ml,0.2ml,0.25ml and 0.3ml of 2.0 microgram/ml (2ppm) of standard solution of phenobarbital were then introduced into each flask and the mixture was diluted to volume of 50ml. The fluorescence of each of these solutions was measured with a fluorometer, which gave values of 3.26, 4.80, 6.41, 8.02, and 9.56 respectively. a) plot the data; b) using the plot from (a) calculate the concentration of phenobarbital in the unknown; c) find the concentration of phenobarbital from the equation in (b).

Explanation / Answer

Concentration in original sample = (1.026 mL)(2.000

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