Determine the identity of the N-terminal amino acid after reconstructing the int

Question

Determine the identity of the N-terminal amino acid after reconstructing the intact protein. The polypeptide was digested with trypsin and, in a separate reaction, it was cleaved with cyanogen bromide.

Cleavage with trypsin yielded 5 peptides that were sequenced by Edman degradation as shown:

1. Ser-Leu

2. Asp-Val-Arg

3. Val-Met-Glu-Lys

4. Ser-Gln-Met-His-Lys

5. Ile-Phe-Met-Leu-Cys-Arg

Cleavage with cyanogen bromide yielded 4 peptides that were sequenced by Edman degradation as shown:

1. His-Lys-Ser-Leu

2. Asp-Val-Arg-Val-Met

3. Glu-Lys-Ile-Phe-Met

4. Leu-Cys-Arg-Ser-Gln-Met

Please give a detailed answer......I dont really understand this at all...

Explanation / Answer

Determine the identity of the N-terminal amino acid after reconstructing the intact protein.

A. Asp ------------ANSWER

Explanation

After reconstructing the intact protein.

Asp-Val-Arg-Val-Met-Glu-Lys-Ile-phe-Met-Leu-Cys-Arg-Ser-Gln-Met-His-Lys-Ser-Leu

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