Select three reasons your beta-carotene would not be the first pigment to eluteo

Question

Select three reasons your beta-carotene would not be the first pigment to eluteoff the column (Only select one of the following answers)

-the sample was not concentrated after extraction, the temp during elution was too high, or someone forgot to place a cotton plug in the bottom of the column

-the spinach leaves were not ground fine enough, the sodium sulfate drying step was skipped, or the sample was concentrated too much after extraction

-you used to incorrect adsorbent during TLC, the elutant was incorrect, or during extraction you discarded the wrong layer

-The elutent was incorrect, the sample was not concentrated after extraction, or sand was not used in the column during column chromatography

Explanation / Answer

Hi

I guess Option C is the answer

the elutent used may be not appropriate for eluting beta carotene or may be the TLC adsorbent used was incorrect ( may be normal phase instead of reversed phase) and there is a possibility of discarding the layer containing beta carotene during extraction which would result in no band of carotene in TLC

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