A main advantage of fluorescence spectroscopy is that emission intensity is dire

Question

A main advantage of fluorescence spectroscopy is that emission intensity is directly related to radiation source intensity. Which of the following experimental changes will lead to a DECREASED intensity of the fluorescence emission observed with a spectrofluorometer? (select all correct options)

Increasing the intensity of the incident radiation

Adding another molecule that has an excitation spectrum that overlaps with the emission wavelength of your analyte.

Spiking the sample solution with a small volume of concentrated analyte solution.

Using narrower slit widths in the excitation monochromator.

Increasing the slit widths of the emission monochromator.

Moving to an excitation wavelength at which the analyte has a lower molar absorptivity.

Increasing the temperature of the sample solution.

Using a solvent that is more viscous.

Increasing the intensity of the incident radiation

Adding another molecule that has an excitation spectrum that overlaps with the emission wavelength of your analyte.

Spiking the sample solution with a small volume of concentrated analyte solution.

Using narrower slit widths in the excitation monochromator.

Increasing the slit widths of the emission monochromator.

Moving to an excitation wavelength at which the analyte has a lower molar absorptivity.

Increasing the temperature of the sample solution.

Using a solvent that is more viscous.

Explanation / Answer

Adding another molecule that has an excitation spectrum that overlaps with the emission wavelength of your analyte.

Moving to an excitation wavelength at which the analyte has a lower molar absorptivity.

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