Aliquots of a 0 . 5-mg / mL standard of BSA are used to construct a standard cur

Question

Aliquots of a 0.5-mg/ mL standard of BSA are used to construct a standard curve for the Bradford protein assay. The tubes contain the following amounts of the BSA solution: 0, 20, 40, 60, 80, and 100 JLL The corresponding absorbencies after adding Bradford reagent are 0, 0.05, 0.09, 0.14, 0.19, and 0.22. If you take 20~L of an unknown and add 80JLL of water, mix, take 10~L of the mixture and add to Bradford reagent, and see an absorbance of 0.08, what is the protein

concentration of the undiluted unknown?

Explanation / Answer

Answer is 33

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