Two different enzymes are able to catalyze the same reaction, A ? B. They both h

Question

Two different enzymes are able to catalyze the same reaction, A ? B. They both have the same Vmax, but differ their Km the substrate A. For enzyme 1, the Km is 1.0 mM; for enzyme 2, the Km is 10 mM. When enzyme 1 was incubated with 0.1 mM A, it was observed that B was produced at a rate of 0.0020 mmoles/minute. a) What is the value of the Vmax of the enzymes? b) What will be the rate of production of B when enzyme 2 is incubated with 0.1 mM A? c) What will be the rate of production of B when enzyme 1 is incubated with 1 M (i.e., 1000 mM) A

Explanation / Answer

a) 0.022 mmol/min;
b) 0.0022 mmol/min;
c) 0.022 mmol/min

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