Which of these high energy groups is transiently transferred to the amino acid i

Question

Which of these high energy groups is transiently transferred to the amino acid in the t- RNA charging reaction? a. ATP b. ADP c. AMP d. Phosphate A protease cleaves proteins on the amino side of Lysine residues. Write the sequence of amino acids that would be released from an elongating ribosome after protease cleavage of this protein: NH2-Met-Gly-Glu-GIn-Lys-Arg His-Ala Trp-COOH a. Met-Gly-Glu-Gin b. Met-Gly-Glu-Gln-Lys c. Lys-Ala-His-Ala-Trp d. Arg-His-Ala-Trp Which of these steps in translation elongation does NOT require GTP hydrolysis? a. Binding of aminoacyl tRNA to the ribosome b. Peptide bond formation c. Translocation d. All of the above Recombinant DNA is created by a. Cloning of genes b. Ligation of separate DNA fragments c. Cleavage by restriction enzymes d. Transfection of plasmids If you transfect cells that contain a wild type Lacz gene on their bacterial chromosome with a blue/white selection plasmid, which color(s) will the resulting bacteria clones have when grown on XGal? a. Only the clones containing a plasmid with an insert will be white b. Only the clones containing a plasmid with an insert will be blue c. All clones will be white d. All clones will be blue

Explanation / Answer

18. c AMP

19. c

20. b. Peptide bond formation.

Binding of aminoacyl tRNA to the ribosome require 1 GTP

Trnaslocation require 1 GTP.

21.a. Cloning of genes.

22.a. Only the clones containing a plasmid with an insert will be white.

Cells transformed with non-recombinant plasmid i.e, only vectors will form blue colonies.

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