To calculate the turnover number of an enzyme, you need to know? A. the enzyme c

Question

To calculate the turnover number of an enzyme, you need to know? A. the enzyme concentration. B. the initial velocity of the catalyzed reaction at [s] > > K_. C. the initial velocity of the catalyzed reaction at low [S]. D. the K_m for the substrate. E. both A and B. Osmosis is movement of a a. polar solute molecule across a membrane. b. charged solute molecule (ion) across a membrane. c. gas molecule across a membrane. d. solvent molecule across a membrane. e. no polar solute molecule across a membrane. Put a cell into a hypotonic solution then a. the cell will expend B. The change in size of the cell cannot be predicted C. The cell's size will remain unchanged D. the cell will shrink Which of the following amino acid are most likely to disrupt as - helix structure? A. Glue. B. Pro. C. Trp. D. Arg. E. Val. Which of the following has layered sheet structure A. -kratin B. collagen C. fibroin D. globins E. none of these Which of the following is the best to describe hemoglobin: A. a domain B. a motif C. a protein with a quaternary structure D. a protein with a tertiary structure E. none of the above

Explanation / Answer

17.Answer is A

Explanation: The following equation is used for calculating the Turnover number

Kcat=Vmax/Et

Kcat=Turnover number

Vmax=Maximum rate of reaction when all the enzyme catalytic sites are saturated with substrate

Et=Total Enzyme concentration

As per the given option only A is fitting.(B is not Vmax it is Vo so the correct option cannot be E)

18. Answer is d

explanation:Osmosis is the net movement of solvent(water) molecule across a semi permeable membrane into a region of higher solute concentration.

19.Answer is B

explanation: Normally animal cells in hypotonic solution will expand.The reason the cell will expand in a hypotonic solution is because the solute concentrations are higher inside the cell and exterior will have more water and this will enter into the cell by osmosis. If the cell takes in excess water that cannot be held inside then the cell will burst.

But, in a plant cell due to the presence of cell wall, the cell does not expand or even burst. Therefore since the question is not stating what type of cell, I am choosing the answer as B.(Pl.Note the first option says'expend'. expend has a dictionary meaning -to use up or spend which I feel cannot be used in the context and so cannot be correct)

20.Answer is B

Explanation:Under normal circumstances the space meant for the back bone of alpha helix, a hydrogen-bonding amide proton, gets jammed by the Proline side chain which has a methylene group .This disrupts the H-bond network and sterics of the helix.

21. Answer is C

Explanation:Fibroin is found in silk. It consists of layered but non parallel beta sheet structure. It is highly tensile.

22. Answer is B

explanation:Hemoglobin has a folded structure formed by a polypetide chain which has 6-8 helices surrounding the heme group and this looks like a motif.

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