Suppose a thin layer of bovine serum albumin (BSA) protein solution was layered

Question

Suppose a thin layer of bovine serum albumin (BSA) protein solution was layered onto the surface of the sample (assume no mixing occurs). You decide to come back when the particle reaches the bottom due to sedimention by gravity. If BSA weighs 66,000 g/mole and has a friction coefficient of 6.81 x 10^-8 g/s, approximately how long would you have to wait? 5. Suppose you had a cell filled with pure water to a height of 1 cm and placed a particle just below the surface of the water. The sample is maintained at 20 degree C

Explanation / Answer

Sedimentation velocity is related to molar mass, M and friction coefficient, f as follows:

S/D = M(1-vr)/RT; D = kT/f; and therefore S = kT/f* M(1-vr)/RT OR

Velocity u/distance x =t. Sedimentation constant S is given by u/x. v is partial specific volume which << 1

Therefore, x/u = t = 1/S = n.f/M = 1*6.81x10-8*6.023x1023/66000 = 6.216x1011 s

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