If I were to say, \" Tumor cells cause changes in cell surface marker protein B

Question

If I were to say, "Tumor cells cause changes in cell surface marker protein B of associated immune cells (TAM’s or tumor-associated macrophages)," what experimental approach would you use to prove this hypothesis? Include:

a) the experimental approach.

b) a general outline of the procedure for that approach, i.e. an overview of how the protocol is conducted, including critical reagents or steps.

c) expected results, including any specific marker proteins associated with the assay that would change or vary.

Nothing too lengthy, please and thank you!

Explanation / Answer

Please find the answers below:

Part a) According to the information, the tumor cells cause change in cell surface marker protein B of associated immne cells, then a simple ELISA assay might be carried out to asertain the quantitative change in the surface protein density of the macrophages. Since ELISA assays are highly specific in nature, one can use anti-cell surface protein B antibody against macrophages to estimate the change in surface protein density of this protein in the target cell. By comparing the results with an experimental positive and negative control, the exact picture of the changes in surface protein B density can be directly evaluated.

Part b) The general steps of ELISA assay can be found as below:

Part c) The expected resuls form ELISA assay are expected to vary with respect to the pipetting errors or any cross contamination only. Otherwise, ELISA is a very robust, quick and powerful tool to evaluate quantiative estimation of analytes.

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